Double-blind trials involving valerian alone and in combination with other herbs have indicated its potential to support both sleep onset and quality. Best-Rest Formula also includes melatonin and GABA. Melatonin is a hormone produced by the pineal gland. It is synthesized in the circadian rhythm by enzymes, which are activated by darkness and depressed by light. GABA is the primary inhibitory neurotransmitter in the brain, and I-Theanine is a bioactive compound in green tea. Suntheanine® is a registered trademark of Taiyo International, Inc.